1 |
4% glutaraldehyde in phosphate buffer |
|
|
50% glutaraldehyde (EM grade) |
8 ml. |
|
Phosphate buffer |
92 ml. |
2 |
Phosphate buffer |
|
|
Solution A : |
|
|
Sodium dihydrogen phosphate monohydrate |
2.26 g. |
|
Distilled water |
100 ml. |
|
Solution B : |
|
|
Sodium hydroxide |
2.52 g. |
|
Distilled water |
100 ml. |
|
Solution C : |
|
|
Glucose (anhydrous) |
5.4 g. |
|
Distilled water |
100 ml. |
|
Solution D : |
|
|
ผสม Solution A 41.5 ml. กับ Solution B 8.5 ml. ปรับ pH 7.3-7.4
|
|
Buffer : |
|
|
ผสม Solution C 5 ml. กับ Solution D 45 ml. เก็บไว้ในตู้เย็น
|
3 |
10% neutral buffer formalin |
|
Sodium dihydrogen phosphate monohydrate |
0.4 g. |
|
Disodium hydrogen phosphate anhydrous |
0.65 g. |
|
40% formaldehyde (100% formalin) |
10 ml. |
|
Distilled water |
90 ml. |